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KMID : 0368420090520060533
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Journal of Plant Biology
2009 Volume.52 No. 6 p.533 ~ p.533
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Diversity of Novel Glutenin Subunits in Bread Wheat (Triticum aestivum L.)
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Kamal Abu Hena Mostafa
Kim Ki-Hyun
Shin Kwang-Hyun
Seo Hyung-Seok
Tsujimoto Hisashi
Heo Hwa-Young
Choi Jong-Soon
Park Chul-Soo
Woo Sun-Hee
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Abstract
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Glutenin is a major determinant of baking performance and viscoelasticity, which are responsible for high-quality bread with a light porous crumb structure of a well-leavened loaf. We analyzed the diversity of glutenin genes from six wheat cultivars (Korean cvs. Keumgang and Jinpum, Chinese cvs. China-108 and Yeonnon-78, and Japanese cvs. Norin-61 and Kantou-107). Glutenins contain two types of isoforms such as high molecular weight glutenin subunit (HMW-GS) and low molecular weight glutenin subunit (LMW-GS). Glutenin fractions were extracted from wheat endosperm using Osborne solubility method. A total of 217 protein spots were separated on two-dimensional gel electrophoresis with isoelectric focusing (wide range of pH 3?10). The proteins spots were subjected to tryptic digestion and identified by matrix assisted laser desorption/ionization?time of flight mass spectrometry. HMW-GS (43 isoforms) and LMW-GS (seven isoforms) are directly responsible for producing high-quality bread and noodles. Likewise, all the seed storage proteins are digested to provide nutrients for the embryo during seed germination and seedling growth. We identified the diverse glutenin subunits in wheat cultivars and compared the gluten isoforms among different wheat cultivars according to quality. This work gives an insight on the quality improvement in wheat crop.
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KEYWORD
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Wheat glutenin subunit, Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), Proteomics, Two-dimensional gel electrophoresis (2-DE)
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